Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00007143" target="_blank" >RIV/00216224:14310/01:00007143 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Detection of prophages in TSST-1-positive <I>Staphylococcus aureus</I> strains by means of specific probes

Original language description

The strains of Staphylococcus aureus producing TSST-1 toxin can induce human disease called Toxic Shock Syndrom (TSS).Production of staphylococcal toxins is based on the function of genes, which are usually localised in chromosome of host bacterium,but sometimes they can be a part of prophage genome.Temperate bacteriophages of S.aureus can move genes by transduction and/or mediate lysogenic conversion of bacterial cells,which leads to increased ability of toxin production, usually of enterotoxins A andE. In this study 25 strains of S.aureus were analysed for presence of prophages of the serological groups A,and F. The presence of prophages was determined using hybridisation by means of specific probes to Sma I and Hin dIII DNA restriction patterns ofS.aureus strains.Probes were prepared from genomic sequences of phages 77 (serological group F),53 (serological group B)and 3A(serological group A). Positive signal in Sma I restriction patterns was found in 92 %strains when probe 77A was

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scripta medica

ISSN

1211-3395

e-ISSN

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Volume of the periodical

74

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

2

Pages from-to

279

UT code for WoS article

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EID of the result in the Scopus database

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