Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from 44 kb SmaI-restriction fragment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00002297" target="_blank" >RIV/00216224:14310/00:00002297 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Identification of Staphylococcus aureus based on PCR amplification of species specific genomic 826 bp sequence derived from 44 kb SmaI-restriction fragment

Original language description

The primers were prepared for PCR-amplification of a part genomic sequence specific to Staphylococcus aureus strains. They were derived from the 44 kb SmaI-restriction fragment of S. aureus genomic DNA (SmaI fragment L on the restriction map of strain NCTC 8325) common to all strains of this species (Pantucek et al. 1996, Int. J. Syst. Bacteriol. 46, 216-222). By hybridization of the labelled 44 kb SmaI-fragment to EcoRI-restriction patterns of the genomic DNA of 13 strains representative of S. aureus subsp. aureus the 2052 bp EcoRI-subfragment was proved to be common to all the tested strains. This fragment was used as the hybridization probe the specificity of which for the species S. aureus was tested in 41 species and subspecies of the genus Staphylococcus as well as in 7 strains of related bacterial genera. The probe hybridized only to the DNA of S. aureus subsp. aureus strains and S. aureus subsp. anaerobius. No hybridization was observed to the DNA of other staphylococcal as wel

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA301%2F99%2FD075" target="_blank" >GA301/99/D075: Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

9th International Symposium on Staphylococci and Staphylococcal Infections

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

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Publisher name

JDS

Place of publication

Kolding

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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