Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00002298" target="_blank" >RIV/00216224:14310/00:00002298 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Preparation of a speciesspecific molecular probe for identification of Staphylococcus aureus

Original language description

In our previous work a 44-kb genomic SmaI-restriction fragment common to all S. aureus strains has been described. This restriction fragment was used as a starting point in preparation of a species-specific hybridisation probe for rapid molecular identification S. aureus strains. EcoRI-restricted genomic DNAs isolated from 13 different S. aureus strains, including the type strain S. aureus CCM 885, and from the type strain S. epidermidis CCM 2124 (negative control) were hybridised to a probe prepared from the whole sequence of the 44-kb fragment. The EcoRI-restriction fragments showing strong hybridisation to the S. aureus 44-kb probe were cloned in the pUCl8 vector in E. coli and used as candidates for the preparation of molecular probes specific forS. aureus. The cloned 2-kb EcoRI-restriction fragment was chosen as the most specific fragment that hybridised to the homologic EcoRl-fragment present in genomic DNA of more than 100 S. aureus strains of different provenance. No hybridisa

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

—

Project

<a href="/en/project/GA301%2F99%2FD075" target="_blank" >GA301/99/D075: Genome-based diagnostics and typing of coagulase-negative staphylococci from human clinical specimens</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Scripta medica

ISSN

1211-3395

e-ISSN

—

Volume of the periodical

72

Issue of the periodical within the volume

8

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

1

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—