Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003302" target="_blank" >RIV/00216224:14310/00:00003302 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of Gentiopicroside in Extracts of Centaurium erythreae and Gentiana lutea by Micellar Electrokinetic Capillary Chromatography

Original language description

Micellar electrokinetic capillary chromatography has been developed as a promising method for the determination of gentiopicroside. The separation conditions have been optimised with respect to the different parameters including SDS concentration, pH andion strength of the background electrolyte, and temperature of capillary. A buffer consisting of 100 mM SDS in 20 mM sodium dihydrogen phosphate, 20 mM disodium tetraborate pH 8.6 was found to be the most suitable electrolyte for this separation. The applied voltage of 28 kV (positive polarity) and the temperature of capillary 20 oC gave the best separation of secoiridiods. The linear detection range for concentration versus peak area for the assay is from 5 to 500 mg . ml-1 (correlation coefficient 0.9998) with a detection limit of 0.3 mg . ml-1. The inter-day reproducibility of the peak area was 2.5 % and the inter-day reproducibility of the migration time was below 0.16 %. The developed method was successfully used to quantify genti

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA303%2F00%2FD062" target="_blank" >GA303/00/D062: Bioavailability and mechanism of antioxidant activity of phenolic acids and lignans</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

J.Liquid Chromatogr. Rel. Technol.

ISSN

1082-6076

e-ISSN

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Volume of the periodical

23

Issue of the periodical within the volume

12

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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