Study of activity of DNase I immobilized on magnetic cellulose

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003589" target="_blank" >RIV/00216224:14310/00:00003589 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Study of activity of DNase I immobilized on magnetic cellulose

Original language description

In our study we concentrated on degradation on both linear, chromosomal and circular plasmid DNAs using immobilized DNase I. A magnetic cellulose support used for immobilization simplifies particle separation from the sample after the reaction. The enzyme was coupled to the particles via the cyanuric chloride method. The effect of DNase I, activated by different metal ions, on hydrolysis of both chromosomal and plasmid DNAs was studied. The activity of DNaseI was estimated from UV spectrophotometrical measurements and confirmed by agarose gel electrophoresis.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

EI - Biotechnology and bionics

OECD FORD branch

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Project

<a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

40th Microsymposium Polymers in Medicine

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

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Publisher name

Institute of Macromolecular Chemistry, CAS

Place of publication

Praha

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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