Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004818" target="_blank" >RIV/00216224:14310/01:00004818 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Influence of metal ions on activity and stability of DNase I immobilized on hydrophilic particles
Original language description
The aim of work has been concentrated on degradation of high-weight and plasmid DNAs using immobilized DNase I. The activity of free and immobilized DNase I was estimated from UV spectrophotometric measurements and from agarose gel electrophoresis. The immobilized enzyme was full actived using Co2+ ions as an activator, a decrease of the activity was observed using other ions. Practical applications of DNase I immobilized on poly(HEMA-co-EDMA) microspheres in cleavage of plasmid DNAs are discussed.
Czech name
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Czech description
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Project
<a href="/en/project/GA203%2F00%2F1339" target="_blank" >GA203/00/1339: Dispersion polymerisation of hydrophilic monomers for the preparation of magnetic carriers used in biotechnological application</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
The 4th Croatian Congress of Food Technologists, Biotechnologists and Nutritionists
ISBN
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ISSN
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e-ISSN
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Number of pages
2
Pages from-to
63
Publisher name
Faculty of Food Technology and Biotechnology, University of Zagreb, Croatia
Place of publication
Zagreb, Croatia
Event location
Zagreb
Event date
Jan 1, 2001
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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