PCR inhibitors in foodstuffs

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003591" target="_blank" >RIV/00216224:14310/00:00003591 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

PCR inhibitors in foodstuffs

Original language description

The polymerase chain reaction (PCR) is a suitable method which enables a rapid identification of microorganisms in foods. However, the presence of inhibitors in the food samples often complicates the identification of the target microorganisms using PCR.The aim of our work was to study the elimination of the inhibitor influence on the identification of target microorganisms in milk and other foods using PCR. As a model microorganism, Salmonella was chosen. Various procedures of the preparation of DNA matrix for PCR were used: pure DNA prepared using the phenol extraction method, boiled cell lysateswith and without immunomagnetic separation of cells. Sensitivity of the PCR reaction was followed in each procedure.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA203%2F98%2F1231" target="_blank" >GA203/98/1231: Oriented immobilization of enzymes and antibodies using biospecific interactions</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

EuroConference- Modern Analytical Methods for Food and Beverage Authentication

ISBN

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ISSN

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e-ISSN

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Number of pages

1

Pages from-to

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Publisher name

Mendel University of Agriculture, Brno

Place of publication

Lednice na Moravě

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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