Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004214" target="_blank" >RIV/00216224:14310/01:00004214 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/01:17013005

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Thermodynamic properties of duplex DNA containing a site-specific d(GpG) intrastrand crosslink formed by an antitumor dinuclear platinum complex

Original language description

Bifunctional polynuclear platinum compounds represent a novel class of metal-based antitumor drugs. A typical agent is [{trans-PtCl(NH3)2}2H2N(CH2)4NH2]Cl2, (1,1/t,t) which coordinates to bases in DNA and forms covalent cross-links. It also forms a 1,2-d(GpG) intrastrand adduct, the equivalent of the major DNA lesion of "classical" cisplatin. In the present study, differential scanning calorimetry and spectroscopic techniques were employed to characterize the influence of this cross-link on the thermalstability and energetics of 20 bp DNA duplexes site-specifically modified by 1,1/t,t. Thermal denaturation data revealed that the cross-link of 1,1/t,t reduced thermal and thermodynamical stability of the duplex noticeably more than that of "classical" cisplatin. The energetic consequences of the intrastrand cross-link at the d(GG) site are discussed in relation to the structural distortions induced by this adduct in DNA and to its recognition and binding by HMG-domain proteins.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

—

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305 1048

e-ISSN

—

Volume of the periodical

29

Issue of the periodical within the volume

10

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

2034

UT code for WoS article

—

EID of the result in the Scopus database

—