Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00003419" target="_blank" >RIV/00216224:14310/01:00003419 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/01:17013004

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

Original language description

The effect of the site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of 20-base pairs DNA duplex is reported. The cross-linked or unplatinated 20-bp duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption and circular dichroism. The cross-link of both platinum isomers increases the thermal stability of the modified duplexes by changing the molecularity of denaturation. The structural perturbation due to the interstrand cross-link of cisplatin increases entropy of the duplex. By contrast, the interstrand cross-link of transplatin is enthalpically almost neutral. These differences are consistent with distinct conformational distortions induced by the interstrand cross-links of the two isomers. It has been proposed that the results of the present work might be associated with distinctly different antitumor effects of these platinum compounds.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

276

Issue of the periodical within the volume

276

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

9655

UT code for WoS article

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EID of the result in the Scopus database

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