Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F05%3A00001323" target="_blank" >RIV/68081707:_____/05:00001323 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)
Original language description
The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum(H) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link,thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in "reduced" (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent.
Czech name
Tepelná stabilita a energetika duplexu DNA 15 párů bází dlouhého obsahujícího meziřetězcový můstek trans-dimmindichloroplatnatého komplexu
Czech description
Byl studován vliv polohy meziřetězcového můstku vytvořeného trans-dimmindichloroplatnatým komplexem (transplatinou) na tepelnou a termodynamickou stabilitu DNA pomocí diferenční skenovací kalorimetrie. Bylo zjištěno, že poloha můstku v duplexu DNA moduluje kooperativitu procesu tání duplexu a následně také tepelnou stabilitu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2005
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biopolymers
ISSN
0006-3525
e-ISSN
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Volume of the periodical
77
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
8
Pages from-to
222-229
UT code for WoS article
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EID of the result in the Scopus database
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