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Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F05%3A00001323" target="_blank" >RIV/68081707:_____/05:00001323 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Thermal stability and energetics of 15-mer DNA duplex interstrand crosslinked by trans-diamminedichloroplatinum(II)

  • Original language description

    The effect of the location of the interstrand cross-link formed by trans-diamminedichloroplatinum(H) (transplatin) on the thermal stability and energetics of 15-mer DNA duplex has been investigated. The duplex containing single, site-specific cross-link,thermodynamically equivalent model structures (hairpins) and nonmodified duplexes were characterized by differential scanning calorimetry, temperature-dependent uv absorption, and circular dichroism. The results demonstrate that the formation of the interstrand cross-link of transplatin does not affect pronouncedly thermodynamic stability of DNA: the cross-link induces no marked changes not only in enthalpy, but also in "reduced" (concentration independent) monomolecular transition entropy. These results are consistent with the previous observations that interstrand cross-links of transplatin structurally perturb DNA only to a relatively small extent.

  • Czech name

    Tepelná stabilita a energetika duplexu DNA 15 párů bází dlouhého obsahujícího meziřetězcový můstek trans-dimmindichloroplatnatého komplexu

  • Czech description

    Byl studován vliv polohy meziřetězcového můstku vytvořeného trans-dimmindichloroplatnatým komplexem (transplatinou) na tepelnou a termodynamickou stabilitu DNA pomocí diferenční skenovací kalorimetrie. Bylo zjištěno, že poloha můstku v duplexu DNA moduluje kooperativitu procesu tání duplexu a následně také tepelnou stabilitu.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    BO - Biophysics

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2005

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Biopolymers

  • ISSN

    0006-3525

  • e-ISSN

  • Volume of the periodical

    77

  • Issue of the periodical within the volume

    4

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    222-229

  • UT code for WoS article

  • EID of the result in the Scopus database