Determination of the Kinetic Parameters of Rhodanese by Electrophoretically Mediated Microanalysis in a Partially Filled Capillary

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00004410" target="_blank" >RIV/00216224:14310/01:00004410 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of the Kinetic Parameters of Rhodanese by Electrophoretically Mediated Microanalysis in a Partially Filled Capillary
Original language description
Recently, a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed, electrophoretically mediated microanalysis (EMMA). In this technique, substrate(s) and enzyme are introduced in the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due the differences in their electrophoretic mobilities. Enzymatic reaction takes place and theresultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected. In this communication the EMMA technique in was applied to determine the kinetic parameters of rhodanese - temperature optimum, KM for thiosulfate and cyanide and type of kinetic mechanism of enzymatic reaction. Rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1) was discovered in 1933 by Lang. It is responsible for the trans
Czech name
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Czech description
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Project
<a href="/en/project/GA525%2F00%2F0785" target="_blank" >GA525/00/0785: Acidification processes in sulphide wastes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Book of abstract 2nd International symposium "Separations in BioSciences SBS 2001"
ISBN
80-7080-437-8
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
112
Publisher name
Czech Chemical Society
Place of publication
Prague
Event location
Prague
Event date
Jan 1, 2001
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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