Electrophoretically Mediated Microanalysis of Rhodanese

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006231" target="_blank" >RIV/00216224:14310/02:00006231 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electrophoretically Mediated Microanalysis of Rhodanese

Original language description

Ten years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method, substrate(s) and enzyme are introduced into the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due to the differences in their electrophoretic mobilities. Enzymatic reactiontakes place and the resultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected.Since its discovery by Bao and Regnier the EMMA methodology has been utilized in anumber of biochemical systems - for assays of enzyme activities, determinations of substrates, Michaelis constants, etc. In this work, the EMMA method was applied to determine the kinetic parameters of rhodanese. Rhodanese (thiosulfate:

Czech name

Electrophoretically Mediated Microanalysis of Rhodanese

Czech description

Ten years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method, substrate(s) and enzyme are introduced into the capillary as distinct plugs, the first analyte injected being the one with the lower electrophoretic mobility. Upon the application of an electric field, the two zones interpenetrate due to the differences in their electrophoretic mobilities. Enzymatic reactiontakes place and the resultant reaction product(s) and the unreacted substrate(s) are electrophoretically transported towards detector, where they are individually detected.Since its discovery by Bao and Regnier the EMMA methodology has been utilized in anumber of biochemical systems - for assays of enzyme activities, determinations of substrates, Michaelis constants, etc. In this work, the EMMA method was applied to determine the kinetic parameters of rhodanese. Rhodanese (thiosulfate:

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F00%2F0785" target="_blank" >GA525/00/0785: Acidification processes in sulphide wastes</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Book of abstracts of Developments in Chromatography and Electrophoresis & Chiranal 2002

ISBN

80-86238-24-5

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

80

Publisher name

Česká společnost chemická - skupina pro chromatografii a elektroforézu

Place of publication

Olomouc

Event location

June 24-27., 2002, Olomouc

Event date

Jan 1, 2002

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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