Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00007524" target="_blank" >RIV/00216224:14310/01:00007524 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Electrochemical impedance spectroscopy of single-stranded and double-helical polynucleotides
Original language description
The dependence of the impedance of the electrode double layer of mercury electrode on frequency around the potentials of the tensammetric peaks of single-stranded and double-helical polynucleotides and DNA was studied. From the frequency dependence of the impedance of the electrode double layer represented in a complex impedance plot (the imaginary component Z'' is plotted against the real component Z', Cole-Cole or Nyquist plot) the electric equivalent circuit of the electrode covered with adsorbed DNAlayer was determined. It was concluded the desorption of denatured ssDNA is accompanied by higher dielectric losses than the desorption of native dsDNA. This can be explained by the higher flexibility of ssDNA compared to the dsDNA. The capacitance peakof single stranded poly A observed at pH 8 around -1.3 V splits at low frequencies in two peaks.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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