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Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00006494" target="_blank" >RIV/00216224:14310/02:00006494 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Use of molecular-biological methods and HPLC for discrimination of Armillaria species in Czech Republic

  • Original language description

    More than 40 isolates containing six species of Armillaria from the collections have been analyzed (A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens) using the PCR of the internal transcribed spacer (ITS). The amplificates were further analysed by RFLP (Alu I, Hinf I and Mbo I) and electrophoreticaly separated both on 3% agarose gel and by IEC- HPLC on TSKgel DEAE-NPR column (Supelco). Digestion with Hinf I allowed discrimination of all six species.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2002

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Book of Abstracts of the congress EFPP

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    7

  • Publisher name

    European Foundation for Plant Pathology

  • Place of publication

    Prague

  • Event location

    8 - 14 September 2002, Prague

  • Event date

    Jan 1, 2002

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article