Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00007137" target="_blank" >RIV/00216224:14310/02:00007137 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Comparison of MRSA-screen latex agglutination, conventional phenotypic methods and <I>mecA</I> gene detection for identification of oxacilin resistance in staphylococci

Original language description

On the whole, 128 staphylococcal isolates (Staphylococcus aureus 65, Staphylococcus epidermidis 42, and Staphylococcus haemolyticus 21 strains) from patients of ten Czech hospitals were classified as susceptible or resistant to oxacillin by the standardbroth dilution micro-method (using the MIC break-point of 4.0 mg/L for resistance). The MRSA-Screen test (Denka Seiken Co., Japan), a latex agglutination for the detection of penicillin binding protein PBP2a in oxacillin/methicillin-resistant staphylococci, was compared with other commonly used phenotypic susceptibility tests. The PCR detection of the mecA gene, the presence of which is equaled to oxacillin resistance, was used as a reference genotypic method. In addition, the determination of the femAgene was performed also by this procedure. All 68 isolates (S. aureus 26, S. epidermidis 21, and S. haemolyticus 21 strains), determined as oxacillin-resistant, contained the mecA gene. The femA gene was present in all these S. aureus str

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia, Section: Cellular and Molecular Biology

ISSN

1335-6399

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

6

Country of publishing house

SK - SLOVAKIA

Number of pages

10

Pages from-to

729

UT code for WoS article

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EID of the result in the Scopus database

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