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The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00007720" target="_blank" >RIV/00216224:14310/02:00007720 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.

  • Original language description

    The electrochemical immunosensor combining three monoclonal antibodies on the same measuring element produced by screen-printing was constructed for the semiquantitative group-specific detection of herbicides belonging to phenoxyalkanoic acids. The combination of different monoclonal antibodies covalently immobilized on three working electrodes and one additional electrode containing immobilized albumin provided a more reliable detection of phenoxyalkanoic acids. Peroxidase-2,4-dichlorophenoxyacetic acid conjugate was used as a tracer suitable for all the antibodies employed in a competitive immunoassay. Several herbicides were used to characterize the immunosensor. The developed immunosensor was tested on samples of surface water spiked with differentherbicides and binary mixtures of herbicides. The proposed approach seems feasible for rapid screening of large series of samples for the presence of herbicides.

  • Czech name

    The four-channel electrochemical immunosensor for detection of herbicides based on phenoxyalkanoic acids.

  • Czech description

    The electrochemical immunosensor combining three monoclonal antibodies on the same measuring element produced by screen-printing was constructed for the semiquantitative group-specific detection of herbicides belonging to phenoxyalkanoic acids. The combination of different monoclonal antibodies covalently immobilized on three working electrodes and one additional electrode containing immobilized albumin provided a more reliable detection of phenoxyalkanoic acids. Peroxidase-2,4-dichlorophenoxyacetic acid conjugate was used as a tracer suitable for all the antibodies employed in a competitive immunoassay. Several herbicides were used to characterize the immunosensor. The developed immunosensor was tested on samples of surface water spiked with differentherbicides and binary mixtures of herbicides. The proposed approach seems feasible for rapid screening of large series of samples for the presence of herbicides.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA525%2F97%2F0924" target="_blank" >GA525/97/0924: Trace analysis of toxic PCB and pesticides using detection and separation methods based on antibody specifity</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2002

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    International Journal of Environmental and Analytical Chemistry

  • ISSN

    0306-7319

  • e-ISSN

  • Volume of the periodical

    83

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    9

  • Pages from-to

    101-109

  • UT code for WoS article

  • EID of the result in the Scopus database