Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008148" target="_blank" >RIV/00216224:14310/03:00008148 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of a disposable electrochemical immunosensor for detection of the herbicide acetochlor

Original language description

The electrochemical immunosensor for the detection of the herbicide acetochlor was developed. Initially, the feasibility of the immunosensor was verified using the standard ELISA technique employing the competitive assay format with immobilized acetochlor. The assay principle of the disposable immunochemical biosensor was similar. The screen-printed electrode system served as the transducer, acetochlor was covalently immobilized on the surface of the gold working electrode activated with a self-assembled monolayer of cystamine. Acetochlor was linked to this layer through S-acetylmercaptosuccinic anhydride (AMSA). A limited amount of anti-acetochlor polyclonal rabbit antibody in solution competed with the analyzed acetochlor and the secondary goat anti-rabbit antibody labeled with peroxidase was chosen for detection. The amperometric measurement of peroxidase activity was carried out using 5?aminosalicylic acid (ASA) and hydrogen peroxide as substrates. Successful regeneration of the se

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA525%2F97%2F0924" target="_blank" >GA525/97/0924: Trace analysis of toxic PCB and pesticides using detection and separation methods based on antibody specifity</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Environmental and Analytical Chemistry

ISSN

0306-7319

e-ISSN

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Volume of the periodical

83

Issue of the periodical within the volume

7-8

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

609-620

UT code for WoS article

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EID of the result in the Scopus database

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