Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14740%2F19%3A00108372" target="_blank" >RIV/00216224:14740/19:00108372 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201900252" target="_blank" >https://onlinelibrary.wiley.com/doi/abs/10.1002/elan.201900252</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.201900252" target="_blank" >10.1002/elan.201900252</a>
Alternative languages
Result language
angličtina
Original language name
Amperometric Immunosensor for Rapid Detection of Honeybee Pathogen Melissococcus Plutonius
Original language description
European foulbrood (EFB) is a honeybee larvae disease caused by a bacterium Melissococcus plutonius. An amperometric immunosensor based on a sandwich assay was developed for rapid point-of-care detection of this pathogen. An in-house made anti-Melissococcus antibody was immobilized to a gold surface of a screen-printed sensor via self-assembled monolayer of cysteamine activated with glutaraldehyde. The direct impedimetric detection of captured microbial cells was tested, however, a better performance was obtained after the formation of sandwich with the peroxidase-labeled antibody in the amperometric mode. The label-free assay was limited by higher non-specific binding. The limit of detection of the immunosensor was 6.6x10(4) CFU mL(-1) (colony-forming units) with wide linear range between 10(5) CFU mL(-1) and 10(9) CFU mL(-1). The whole analysis was completed within 2 h, which is shorter compared to common laboratory diagnostic tools, such as enzyme-linked immunosorbent assay or polymerase chain reaction. Furthermore, atomic force microscopy was used for confirmation of the bacteria presence on the electrode surface. The developed immunosensor was successfully employed in the analysis of real samples of honeybees and larvae. The achieved results demonstrate the potential of the amperometric immunosensor for practical in-field diagnosis of EFB, which can prevent infection spreading and connected losses of honeybee colonies.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electroanalysis
ISSN
1040-0397
e-ISSN
1521-4109
Volume of the periodical
31
Issue of the periodical within the volume
10
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
1969-1976
UT code for WoS article
000474184300001
EID of the result in the Scopus database
2-s2.0-85068607764