Development of the piezoelectric biosensor for acetochlor detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00009629" target="_blank" >RIV/00216224:14310/03:00009629 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Development of the piezoelectric biosensor for acetochlor detection

Original language description

The piezoelectric immunosensor for the determination of acetochlor was developed. The surface of gold electrodes of piezoelectric quartz crystals was modified by self-assembled thiolayers using either 4-aminothiophenol or dithiobis(succinimidyl propionate). In the next step, the modified surface was used for the coupling of acetochlor-protein conjugates. Acetochlor was conjugated to ovalbumin using either thiopropionic acid or acetylthiosuccinimidyl anhydride (AMSA). The acetochlor-modified crystals were used for characterization of the anti-acetochlor polyclonal antibody (Ab). The kinetic rate and equilibrium constants were compared for both types of immobilization. For acetochlor immobilized through AMSA, the dissociation rate constant was 20-times lower. The possibility of using this system for the competitive determination of free acetochlor in water was further studied. The detection limit (10% decrease of relative binding of the antibody) was 0.20 mg/l). The piezoelectric crystal

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/OC%20518.30" target="_blank" >OC 518.30: Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical Letters

ISSN

0003-2719

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

10

Country of publishing house

RU - RUSSIAN FEDERATION

Number of pages

15

Pages from-to

2443-2457

UT code for WoS article

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EID of the result in the Scopus database

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