Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010106" target="_blank" >RIV/00216224:14310/04:00010106 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Piezoelectric biosensors for real-time monitoring of hybridization and detection of the hepatitis C virus

Original language description

The piezoelectric quartz crystal resonators modified with oligonucleotide probes were used for detection of hepatitis C virus in serum. The gold electrodes on either rough or smooth surface crystals were modified with a self-assembled monolayer of cystamine. After activation with glutaraldehyde, either avidin or streptavidin were immobilized and used for attachment of biotinylated DNA probes (4 different sequences). Thus prepared piezoelectric biosensors were used in a flow-through setup for direct monitoring of DNA resulting from the reverse transcriptase linked polymerase chain reaction (RT-PCR) amplification of the original viral RNA. The samples of patients with hepatitis C were analyzed and the results were compared with the standard RT-PCR procedure (Amplicor test kit of Roche, microwell format with spectrophotometric evaluation). The piezoelectric hybridization assay was completed in 10 min and the same sensing surface was suitable for repeated use.

Czech name

Piezoelektrický biosensor pro sledování hybridizace v reálném čase a pro stanovení viru hepatitidy C

Czech description

Piezoelektrický krystalový resonátor byl modifikován oligonukleotidovou probou a použit pro detekci virové nukleové kyseliny v seru. Biotinylované proby byly navázany na monovrstvu avidinu kovalentně vázanou na povrch zlatých elektrod. Virová RNA byla pomocí RT-PCR techniky převedena na DNA, které pak byly detekovány PZ biosensorem. Pro srovnání byly vzorky analyzovány také soupravou Amplicor firmy Roche (fotometrické stanovení na mikrodestičce).

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/OC%20518.30" target="_blank" >OC 518.30: Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Virological Methods

ISSN

0166-0934

e-ISSN

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Volume of the periodical

117

Issue of the periodical within the volume

2

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

145-151

UT code for WoS article

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EID of the result in the Scopus database

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