The effect of cultivation conditions on protein composition of Paracoccus denitrificans membrane

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F02%3A00019686" target="_blank" >RIV/00216224:14310/02:00019686 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The effect of cultivation conditions on protein composition of Paracoccus denitrificans membrane

Original language description

New method using two-dimensional gel electrophoresis was optimised for Paracoccus denitrificans cell envelope study. This approach consist of sample preparation especially optimised for Paracoccus denitrificans membrane proteins, carrier-ampholyte isoelectric focusation in the first dimension (CA-IEF) and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) in the second dimension. Acquired 2-D gels were compared using image analysis and PDQUEST software. Various conditions of initial solubilisation using various detergents were tested. Sodium dodecyl sulphate (SDS), 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfo-nate (CHAPS), dodecyl-â-D-maltoside and 6-aminocaproic acid were used for this purpose. Sample buffer and IEF gel composition effects on 2-D gels resolution were also tested. Extraction using free detergent was compared with extraction using diluted detergent in mixture with sample buffer. Dilution ratio between presolubilised extract and sample buffer

Czech name

Vliv kultivačních podmínek na proteinové složení membránové frakce Paracoccus denitrificans

Czech description

Byla optimalizována nová metoda k analýze membránové frakce bakterie Paracoccus denitrificans za použití isoelektrické fokusace s nosnými amfolyty a dodecylsulfátové polyakrylamidové elektroforézy. Při optimalizaci byly testovány různé postupy přípravy vzorku za použití různých detergentů. 2-D gely byly vyhodnocovány pomocí software PDQUEST.

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA203%2F01%2F1589" target="_blank" >GA203/01/1589: Mechanisms involved in regulation of respiratory systems in denitrification bacteria by environmental factors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2002

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

VI. Pracovní setkání biochemiků a molekulárních biologů. Sborník příspěvků.

ISBN

80-210-2777-0

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

67

Publisher name

Masarykova univerzita

Place of publication

Brno

Event location

7.2.2002 Brno

Event date

Jan 1, 2002

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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