Inhibition study of rhodanese by means of electrophoretically mediated microanalysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00007945" target="_blank" >RIV/00216224:14310/03:00007945 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Inhibition study of rhodanese by means of electrophoretically mediated microanalysis
Original language description
A combination of the electrophoretically mediated microanalysis methodology with a partial filling technique was applied for the inhibition study of the bovine liver rhodanese by 2-oxoglutarate. In this set-up, the part of capillary is filled with the buffer best for the enzymatic reaction whereas, the rest of the capillary is filled with the background electrolyte optimal for separation of substrates and products. The estimated value of KI for 2-oxoglutarate was 3.62 x 10-4 ą 1.43 x 10-4 M with respectto cyanide and 1.40 x 10-3 ą 1.60 x 10-4 M with respect to thiosulfate. Moreover the type of inhibition was also evaluated. The findings of 2-oxogluatarate as the competitive inhibitor with respect to cyanide and as the uncompetitive inhibitor with respect to thiosulfate are in accordance with previous literature data.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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