Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00015670" target="_blank" >RIV/00216224:14310/06:00015670 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determination of cyanide in microliter samples by CE using on-capillary enzymatic reaction with rhodanese
Original language description
Thirteen years ago a new application for the evaluation of enzymatic reactions in capillary electrophoresis was proposed and developed, electrophoretically mediated microanalysis (EMMA). In this method the capillary is used not only as a separation medium but also as a reaction chamber. Since its discovery by Bao and Regnier [1], the EMMA methodology has been utilized in a number of biochemical systems [2,3]. In this work, the EMMA method was applied to determine cyanide after its on-capillary enzymaticreaction with rhodanese (thiosulfate: cyanide sulfur transferase, EC 2.8.1.1).Consequnetly cyanide thus could be determine as thiocyanate with adequate sensitivity by means of CE with simple UV detection. Compared to other assays, the EMMA method is rapid, can be automated, and requires only small amount of samples.
Czech name
Stanovení kyanidu v ul vzorcích pomocí CE a on-line reakce s rhodanasou.
Czech description
Byla vypracována metoda pro stanovení kyanidu v ul vzorcích pomocí CE a on-line reakce s rhodanasou.

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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