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Comparison of on-capillary derivatization of amino acids using TDLFP and EMMA mixing of reactants for capillary electrophoresis coupled with LIF detection

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088403" target="_blank" >RIV/00216224:14310/16:00088403 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Comparison of on-capillary derivatization of amino acids using TDLFP and EMMA mixing of reactants for capillary electrophoresis coupled with LIF detection

  • Original language description

    Amino acids are very important compounds for living organisms. They exhibit low or no native fluorescence, thus derivatization is essential for their sensitive determination by laser induced fluorescence detection. Capillary electrophoresis offers conducting the derivatization reaction in pre-capillary, on capillary or post capillary arrangement. In the case of on-capillary derivatization, mixing of the reactants can be performed by longitudinal diffusion, by transverse diffusion of laminar flow profiles (TDLFP), or by electrophoretically mediated microanalysis (EMMA). This contribution deals with optimization of EMMA for derivatization of all standard AAs using naphthalene-2,3-dicarboxaldehyde and sodium cyanide and comparison with derivatization of AAs using TDLFP methodology.

  • Czech name

  • Czech description

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    13th International Interdisciplinary Meeting on Bioanalysis (CECE2016)

  • ISBN

    9788090495944

  • ISSN

  • e-ISSN

  • Number of pages

    4

  • Pages from-to

    100-103

  • Publisher name

    Institute of Analytical Chemistry of the CAS, v. v. i., Brno, Czech Republic

  • Place of publication

    Brno, Czech Republic

  • Event location

    Brno, Czech Republic

  • Event date

    Jan 1, 2016

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article