Comparison of on-capillary derivatization of amino acids using TDLFP and EMMA mixing of reactants for capillary electrophoresis coupled with LIF detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00088403" target="_blank" >RIV/00216224:14310/16:00088403 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Comparison of on-capillary derivatization of amino acids using TDLFP and EMMA mixing of reactants for capillary electrophoresis coupled with LIF detection
Original language description
Amino acids are very important compounds for living organisms. They exhibit low or no native fluorescence, thus derivatization is essential for their sensitive determination by laser induced fluorescence detection. Capillary electrophoresis offers conducting the derivatization reaction in pre-capillary, on capillary or post capillary arrangement. In the case of on-capillary derivatization, mixing of the reactants can be performed by longitudinal diffusion, by transverse diffusion of laminar flow profiles (TDLFP), or by electrophoretically mediated microanalysis (EMMA). This contribution deals with optimization of EMMA for derivatization of all standard AAs using naphthalene-2,3-dicarboxaldehyde and sodium cyanide and comparison with derivatization of AAs using TDLFP methodology.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP206%2F12%2FG014" target="_blank" >GBP206/12/G014: Center for advanced bioanalytical technologies</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
13th International Interdisciplinary Meeting on Bioanalysis (CECE2016)
ISBN
9788090495944
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
100-103
Publisher name
Institute of Analytical Chemistry of the CAS, v. v. i., Brno, Czech Republic
Place of publication
Brno, Czech Republic
Event location
Brno, Czech Republic
Event date
Jan 1, 2016
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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