Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F14%3A00074190" target="_blank" >RIV/00216224:14310/14:00074190 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Optimization of amino acid derivatization procedure using naphthalene-2,3-dicarboxaldehyde for capillary electrophoresis with fluorescence detection
Original language description
Amino acids are very important compounds in living organisms and their quantification in biological fluids can be used for diagnostic purposes. For determination of amino acids by capillary electrophoresis coupled with laser induced fluorescence detection, derivatization procedure is necessary because only a few amino acids exhibit native fluorescence. Derivatization reagent naphthalene-2,3-dicarboxaldehyde reacts with primary amines in the presence of nucleophile, such as cyanide anion, to form fluorescent derivates. Derivatization reaction can be done in pre-capillary or on-capillary arrangement. In on-capillary arrangement the capillary is used both for separation and derivatization. Optimization of pre-capillary and on-capillary derivatization procedure of amino acids using naphthalene-2,3-dicarboxaldehyde and NaCN, and comparison of these approaches is subject of this contribution.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
11 th International Interdisciplinary Meeting on Bioanalysis CECE 2014
ISBN
9788090495920
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
154-157
Publisher name
Institute of Analytical Chemistry AS CR, v. v. i., Brno, Česká republika
Place of publication
Brno
Event location
Brno
Event date
Jan 1, 2014
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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