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MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F16%3A00087859" target="_blank" >RIV/00216224:14310/16:00087859 - isvavai.cz</a>

  • Alternative codes found

    RIV/65269705:_____/16:00066191

  • Result on the web

    <a href="http://dx.doi.org/10.1002/elps.201500587" target="_blank" >http://dx.doi.org/10.1002/elps.201500587</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/elps.201500587" target="_blank" >10.1002/elps.201500587</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    MEKC LIF method for analysis of amino acids after on-capillary derivatization by transverse diffusion of laminar flow profiles mixing of reactants for assessing developmental capacity of human embryos after in vitro fertilization

  • Original language description

    Evaluating the physiological state of an organism is of clinical importance. In assisted reproduction, knowledge of the embryo’s physiology is crucial for selecting the embryo with the highest developmental capacity to ensure high pregnancy rates. Amino acids (AAs) are involved in many biochemical processes during embryo development, which means that the determination of AA fluctuations in the embryo’s surroundings can determine the embryo’s physiological state. Since current embryo selection methods are mainly based on visual assessment, which lacks proper accuracy, a novel method for the analysis of AAs in the embryo’s surroundings was developed. AAs were analyzed by means of MEKC-LIF after on capillary derivatization by naphthalene 2,3 dicarboxaldehyde. The reactants were injected under the three zone arrangement and mixed using the transverse diffusion of laminar flow profiles methodology.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CE - Biochemistry

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GAP206%2F11%2F0009" target="_blank" >GAP206/11/0009: Capillary electrophoresis as a member of the metabolomic analytical toolbox</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2016

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electrophoresis

  • ISSN

    0173-0835

  • e-ISSN

  • Volume of the periodical

    37

  • Issue of the periodical within the volume

    17-18

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    8

  • Pages from-to

    2305-2312

  • UT code for WoS article

    000383596800004

  • EID of the result in the Scopus database