Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00007946" target="_blank" >RIV/00216224:14310/03:00007946 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Determination of homocysteine in human plasma by mekc and in-capillary detection reaction with 2,2-dipyridyldisulfide
Original language description
In this paper, we present a new method for homocysteine quantitation in human plasma based on in-capillary reaction of homocysteine with 2,2-dipyridyldisulfide. Homocysteine is in this so called thiol-exchange reaction quantitatively transformed in mixeddisulfide concomitantly with formation of equimolar amount of 2-thiopyridone that is further separated by MEKC and determined specifically at 343 nm. The concentration of homocysteine is thus estimated indirectly from the result of 2-thiopyridone determination. The linear detection range for concentration versus peak area for the assay was from 0.03 to 3 mM (correlation coefficient 0.994) with a detection limit of 6 mM and a limit of quantitation 20 mM. The inter-day reproducibility of the peak area and the migration time were 1.37 % and 0.05 %, respectively. The method is simple, relatively rapid and can be easily automated. Moreover the common CE apparatus with UV detector can be used to distinguish between normal and pathological hy
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography A
ISSN
0021-9606
e-ISSN
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Volume of the periodical
990
Issue of the periodical within the volume
(1+2)
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
197
UT code for WoS article
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EID of the result in the Scopus database
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