Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008112" target="_blank" >RIV/00216224:14310/03:00008112 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Square wave voltammetric analysis of azidothymidine in the presence of oligonucleotides and chromosomal DNA
Original language description
Azidothymidine ( AZT, Zidovudine) is a synthetic nucleoside analogue with activity against human immunodeficiency virus type 1 (HIV). Recently, our working group has developed and optimised a square wave voltammetric method for analysis of AZT in biological samples. The aim of the study is the determination of AZT in the presence of synthetic oligonucleotides (ODNs) and calf thymus ssDNA or dsDNA on the hanging mercury drop electrode. Both AZT and DNA yield voltammetric signals on the mercury electrode;AZT provides an irreversible reduction peak at the potential near -1.1 V (vs. Ag/AgCl/3M KCl) and DNA provides a reduction peak of adenine and cytosine at the potential ca. -1.4 V. Square wave voltammetric signals of AZT and DNA were measured in phosphate buffer (pH 8.0) at a frequency of 2000 Hz and accumulation time of 10 s. We found that AZT peak height is not affected by the presence of DNA and therefore we assume that electron transfer proceeds through the adsorption layer of ssDNA
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CG - Electrochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XVII th International Symposium on Bioelectrochemictry and Bioenergetics
ISBN
nebylo uděleno
ISSN
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e-ISSN
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Number of pages
1
Pages from-to
139
Publisher name
The Bioelectrochemical Society
Place of publication
Florence, Italy
Event location
Florence, Italy
Event date
Jun 19, 2003
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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