Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008229" target="_blank" >RIV/00216224:14310/03:00008229 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/03:17033006

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electrochemical Study of Heavy Metals and Metallothionein in Yeast Yarrowia lipolytica

Original language description

The bioaccumulation of heavy metals (cadmium, nickel, cobalt and zinc) and the effect of these metals on the production of metallothionein and metallothionein-like proteins (MT) in Yarrowia lipolytica was studied by electrochemical methods. The concentrations of heavy metals were determined by the differential pulse voltammetry (DPV). A combination of the constant current chronopotentiometric stripping analysis (CPSA) and adsorptive transfer stripping technique (AdTS) was used to determine the content of MT in cells. Both the bioaccumulation of heavy metals and the production of MT in different cell compartments of Yarrowia lipolytica exposed to heavy metals were monitored. The LD50 of each metal was determined from the number of viable cells in yeastcultures. The highest concentrations of heavy metals were found in the cell wall and membrane debris while the lowest concentrations were detected in cytoplasm. Cadmium and nickel showed the most significant effect on the production of MT

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

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Volume of the periodical

neuveden

Issue of the periodical within the volume

60

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

29-36

UT code for WoS article

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EID of the result in the Scopus database

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