Study of metallothionein modified electrode surface behaviour in the presence of heavy metal ions - biosensor

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F05%3A00000273" target="_blank" >RIV/62156489:43210/05:00000273 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Study of metallothionein modified electrode surface behaviour in the presence of heavy metal ions - biosensor

Original language description

An increasing concentration of heavy metals in the environment is a serious problem for human and animal health protection and production of foodstuffs in many countries around the world. The aim of this paper was to suggest a new heavy metal biosensor based on interaction of metals (cadmium and zinc) with metallothionein, which belongs to group of intracellular, high molecular and cysteine-rich proteins binding heavy metals, using adsorptive transfer stripping (AdTS) differential pulse voltammetry (DPV). Primarily, we studied the electrochemical behaviour of MT on the surface of hanging mercury drop electrode by AdTS DPV. Perfect coverage of the electrode surface -- forming of the surface assembled monolayer -- was probably reached at time about 240 sfor 10 mM protein concentration. The detection limits of the selected heavy metals (cadmium and zinc), which were analysed in the presence of the basic electrolyte -- 0.5 M NaCl (pH 6.4), were 250 fmole and 350 fmole in 5 ml drop, respec

Czech name

Studium metalothioneinem modifikovaného provrchu elektrody v přítomnosti těžkých kovů-biosenzor

Czech description

An increasing concentration of heavy metals in the environment is a serious problem for human and animal health protection and production of foodstuffs in many countries around the world. The aim of this paper was to suggest a new heavy metal biosensor based on interaction of metals (cadmium and zinc) with metallothionein, which belongs to group of intracellular, high molecular and cysteine-rich proteins binding heavy metals, using adsorptive transfer stripping (AdTS) differential pulse voltammetry (DPV). Primarily, we studied the electrochemical behaviour of MT on the surface of hanging mercury drop electrode by AdTS DPV. Perfect coverage of the electrode surface -- forming of the surface assembled monolayer -- was probably reached at time about 240 sfor 10 mM protein concentration. The detection limits of the selected heavy metals (cadmium and zinc), which were analysed in the presence of the basic electrolyte -- 0.5 M NaCl (pH 6.4), were 250 fmole and 350 fmole in 5 ml drop, respec

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GP525%2F04%2FP132" target="_blank" >GP525/04/P132: Study of protection mechanisms for stress-induced by heavy metals</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

18

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1649-1657

UT code for WoS article

—

EID of the result in the Scopus database

—