Searching for plant telomere-binding proteins using genomic and proteomic approaches

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008350" target="_blank" >RIV/00216224:14310/03:00008350 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Searching for plant telomere-binding proteins using genomic and proteomic approaches

Original language description

Two proteins, AtTBP2 (acc. nos. T46051 (protein database), GI:638639 (nucleotide database)) and AtTBP3 (BAB08466, GI:9757879) from Arabidopsis thaliana, were found in the database on the basis of amino acid sequence similarity with telomere-binding protein PcMyb1 (acc. No. T15038) from Petroselinum crispum. Both of the proteins were expressed in various A. thaliana tissues and as the PcMyb2 contain Myb-like domain at the N-terminus which enables the double-stranded DNA binding and additionally a histoneH1/H5-like DNA-binding domain in the middle of the protein sequence. The cDNAs of ATTB1 and ATTB2 were cloned and the experiments carried out in Saccharomyces cerevisiae using the two-hybrid system showed interaction between the two proteins and self-interaction of each of the proteins.

Czech name

Hledání rostlinných telomer-vazebných proteinů prostřednictvím genomického a proteomického přístupu

Czech description

Two proteins, AtTBP2 (acc. nos. T46051 (protein database), GI:638639 (nucleotide database)) and AtTBP3 (BAB08466, GI:9757879) from Arabidopsis thaliana, were found in the database on the basis of amino acid sequence similarity with telomere-binding protein PcMyb1 (acc. No. T15038) from Petroselinum crispum. Both of the proteins were expressed in various A. thaliana tissues and as the PcMyb2 contain Myb-like domain at the N-terminus which enables the double-stranded DNA binding and additionally a histoneH1/H5-like DNA-binding domain in the middle of the protein sequence. The cDNAs of ATTB1 and ATTB2 were cloned and the experiments carried out in Saccharomyces cerevisiae using the two-hybrid system showed interaction between the two proteins and self-interaction of each of the proteins.

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F02%2F0027" target="_blank" >GA204/02/0027: Molecular analysis of the structure and function of typical and alternative telomeres in plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

VII. Pracovní setkání biochemiků a molekulárních biologů

ISBN

80-210-3053-4

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

27

Publisher name

Masarykova univerzita v Brně

Place of publication

Brno

Event location

Brno

Event date

Jan 29, 2003

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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