Characterisation of two Arabidopsis thaliana myb-like proteins showing affinity to telomeric DNA sequence

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008351" target="_blank" >RIV/00216224:14310/03:00008351 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Characterisation of two Arabidopsis thaliana myb-like proteins showing affinity to telomeric DNA sequence

Original language description

Using a genomic approach, two Arabidopsis thaliana genes coding for candidate myb-like telomere bnding proteins were cloned and expressed in E. coli. Both proteins, termed as AtTBP2 and AtTBP3, contain a single Myb-like DNA-binding domain at the N-terminus, and a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. Gel-retardation assays showed that each of the two proteins is able to bind G-rich strand and double-strand DNA of plant telomeric sequence with the affinity proportional to a number of telomeric repeats.

Czech name

Charakterizace dvou myb-like proteinů z Arabidopsis thaliana, které vykazují afinitu k telomerickým sekvencím

Czech description

Using a genomic approach, two Arabidopsis thaliana genes coding for candidate myb-like telomere bnding proteins were cloned and expressed in E. coli. Both proteins, termed as AtTBP2 and AtTBP3, contain a single Myb-like DNA-binding domain at the N-terminus, and a histone H1/H5-like DNA-binding domain in the middle of the protein sequence. Gel-retardation assays showed that each of the two proteins is able to bind G-rich strand and double-strand DNA of plant telomeric sequence with the affinity proportional to a number of telomeric repeats.

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA204%2F02%2F0027" target="_blank" >GA204/02/0027: Molecular analysis of the structure and function of typical and alternative telomeres in plants</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2003

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

VII. Pracovní setkání biochemiků a molekulárních biologů

ISBN

80-210-3053-4

ISSN

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e-ISSN

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Number of pages

1

Pages from-to

33

Publisher name

Masarykova univerzita v Brně

Place of publication

Brno

Event location

Brno

Event date

Jan 29, 2003

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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