Development of piezoelectric immunosensors for competitive and direct determination of atrazine

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00008696" target="_blank" >RIV/00216224:14310/03:00008696 - isvavai.cz</a>
Alternative codes found
RIV/00000493:G41__/03:25150
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Development of piezoelectric immunosensors for competitive and direct determination of atrazine
Original language description
The improved highly sensitive piezoelectric immunosensor has been developed. The gold electrodes of the piezoelectric quartz crystals were modified with self-assembled layer of cysteamine and carboxyderivative of atrazine was covalently attached after activation with O-(N-succinimidyl)-N,N,N',N'-tetramethyluronium tetrafluoroborate (TSTU). The competitive immunoassay for atrazine was developed using anti-atrazine monoclonal antibody (MAb) D6F3. The limit of detection for atrazine was 0.025 ng/ml, the total time of analysis being 25 min. The repeated use of the immunosensors was possible after regeneration using pepsin at pH 2.0. The direct immunosensor for atrazine was constructed by oriented immobilization of anti-atrazine MAb to Protein A covalentlyattached to the gold surface activated with 3,3'-dithio-bis(propionic acid N-hydroxysuccinimide ester) (DTSP). The MAb-Protein A complex was stabilized by cross-linking with dimethyl pimelimidate. Thus developed immunosensor was able to s
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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