Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F06%3A00016802" target="_blank" >RIV/00216224:14310/06:00016802 - isvavai.cz</a>
Alternative codes found
RIV/00000493:G41__/05:25151
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Piezoelectric immunosensors for polychlorinated biphenyls operating in aqueous and organic phases
Original language description
The piezoelectric quartz crystal operating in the active mode was used as a transducer for construction of immunosensors for determination polychlorinated biphenyls(PCB). An apparatus for flow-through measurements with piezosensors in pure organic phasewas constructed. The anti-PCB polyclonal antibody was immobilized in an oriented way via protein A on a smooth gold surface. The antibody high cross-reactivity for 2,4,4'-trichlorobiphenyl, 4,4'-dichlorobiphenyl and 3,3,4,4'-tetrachlorbiphenyl allowed for a specific determination of these congeners in samples. PCBs were determined either directly or using competition with a tracer, synthesized from polystyrene particles by covalent attachment of 4,4'-dichlorobiphenyl (DCB). The direct piezoelectric immunosensor operated in phosphate buffer with 5% dimethylsulphoxide. It was possible to detect coplanar PCBs in the range 1-50ppm. A higher detection limit is compensated here by the low cost and short time of analysis, below 20 min. The sam
Czech name
Piezoelektrický imunosensor pro polychlorované bifenyly fungující ve vodném prostředí i v organických rozpouštědlech
Czech description
Byl vyvinut piezoelektrický imunosensor s imobilizovanou protilátkou specifickou vůči polychlorovaným bifenylům. Sensor umožňuje přímé i kompetitivní měření PCB, je možné pracovat v nevodném prostředí, což je zvláště vhodné pro nepolární látky.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/OBVTUO2003001" target="_blank" >OBVTUO2003001: Imunosensors of CWA and BWA</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Sensors and Actuators B Chemical
ISSN
0925-4005
e-ISSN
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Volume of the periodical
113
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
900-910
UT code for WoS article
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EID of the result in the Scopus database
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