Moleular cloning and overexpression of bacterial lectins from human pathogen Pseudomonas aeruginosa involved in host-oligosaccharide recognition. Thermodynamical characterisation of lectin-monosaccharides interactions

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F03%3A00009310" target="_blank" >RIV/00216224:14310/03:00009310 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
Moleular cloning and overexpression of bacterial lectins from human pathogen Pseudomonas aeruginosa involved in host-oligosaccharide recognition. Thermodynamical characterisation of lectin-monosaccharides interactions
Original language description
Human pathogenic bacteria Pseudomonas aeruginosa colonize patients with a number of chronic lung diseases and is a major cause of morbidity and mortality in cystic fibrosis patients. Specific oligosaccharide-mediated recognition and adhesion are key points in the initiating steps of the P. aeruginosa infection. The bacterium produces two sugar-recognising proteins PA-IL (LecA) and PA-IIL (LecB), galactose- and fucose-binding lectins respectively, that can play a crucial role in adhesion and specific recognition of a host by the pathogen and contribute to its virulence. Recently, the crystal structure of PA-IIL complexed with fucose has been solved at 1.3 A[1]. P. aeruginosa expresses these lectins under stressed conditions that limit their in vitro production for further structure-functional characterisation. Therefore, both lecA and lecB genes were cloned into pET25 vector, expressed in E.coli BL21(DE3) cells as host organism and purified by affinity chromatography on Sepharose 4B and
Czech name
—
Czech description
—

Project
<a href="/en/project/ME%20675" target="_blank" >ME 675: Structural-functional study of bacterial viruses lectins.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
European Congress of Young Chemists
ISBN
—
ISSN
—
e-ISSN
—
Number of pages
1
Pages from-to
70
Publisher name
Chemical Scientific Set "Flogiston", Warsaw University of Technology
Place of publication
Warsaw
Event location
Zakopane
Event date
Jan 1, 2003
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
—

Similar results(10)