Pseudomonas aeruginosa lectin PA-IIL: cloning, expression, mutation, crystallisation and thermodynamic characterisation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010509" target="_blank" >RIV/00216224:14310/04:00010509 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Pseudomonas aeruginosa lectin PA-IIL: cloning, expression, mutation, crystallisation and thermodynamic characterisation

Original language description

Pseudomonas aeruginosa is an opportunistic pathogen. Chronic lung colonisation by the bacterium is the major cause of morbidity and mortality in cystic fibrosis patients. Identification of the molecules involved in this pathogen specific recognition of the modified host cell components is of therapeutic interest. P. aeruginosa produces high levels of D galactose and L-fucose binding lectins, PA-IL (LecA) and PA-IIL (LecB) respectively, which are associated with its cytotoxic virulence and could be involved in primary recognition of the host organism and in biofilm formation [1]. For further structure-functional characterisation, the lecB gene was cloned into pET25 vector, the recombinant protein was expressed in E. coli BL21(DE3) cells and purified byaffinity chromatography. Isothermal titration microcalorimetry experiments confirmed unusually high affinity of the PA-IIL lectin for monosaccharides (KA = 0.3 x 106 M-1 for L-fucose). Crystal structure of the PA-IIL/fucose complex reveal

Czech name

PA-IIL lektin z Pseudomonas aeuruginosa:klonování, exprese, mutageneze, krystalizace a termodynamická charakterizace

Czech description

PA-IIL lektin z Pseudomonas aeuruginosa:klonování, exprese, mutageneze, krystalizace a termodynamická charakterizace

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Biochemistry

ISSN

0014-2956

e-ISSN

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Volume of the periodical

Supplement

Issue of the periodical within the volume

S1

Country of publishing house

PL - POLAND

Number of pages

2

Pages from-to

154-155

UT code for WoS article

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EID of the result in the Scopus database

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