Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010027" target="_blank" >RIV/00216224:14310/04:00010027 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43410/04:00002149

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Variations in ITS of ribosomal DNA of Czech Armillaria species determined by PCR and high performance liquid chromatography

Original language description

We analysed 40 isolates of species A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae and A. tabescens mostly collected in Czech Republic by PCR-RFLP.Restriction fragments were analysed by ion-exchange high performance liquid chromatography that proved to be more useful, more sensible and less time consuming than classical electrophoresis on agarose gel.The cluster analysis based on the restrictions patterns of restriction endonucleases AluI and HinfI divided the analysed species into three groups.

Czech name

Determinace variability ITS regionu v rámci ribozomální DNA václavek pomocí PCR a HPLC

Czech description

Analyzovali jsme celkem 40 izolátů druhů A. borealis, A. cepistipes, A. gallica, A. mellea, A. ostoyae a A. tabescens pomocí PCr a následné restrikční anlýzy. Restrikční fragenty byly analyzovány pomocí HPLC kolony, což zvýšilo senzitivitu, přesnost, rychlost a snížilo cenu detekce.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Mycological Research

ISSN

0953-7562

e-ISSN

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Volume of the periodical

108

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

1153

UT code for WoS article

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EID of the result in the Scopus database

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