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Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00019893" target="_blank" >RIV/00216224:14310/04:00019893 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Identification of bifidobacteria by polymerase chain reaction and amplified ribosomal DNA restriction analysis

  • Original language description

    Two molecular methods were applied to verify the species identification of Bifidobacterium isolates from the Culture Collection of Dairy Microorganisms (CCDM, Tábor, ČR), previously identified by fenotypic techniques only. The methods used for identification in this study were the genus and species specific PCR and restriction analysis of amplified ribosomal DNA restriction analysis with three restriction enzymes. The methods were introduced and optimalised using type and reference strains of Bifidobacterium species. The molecular methods chosen enabled species identification of the analysed strains and provided congruent results.

  • Czech name

    Identifikace bifidobakterií s využitím metod PCR a ARDRA

  • Czech description

    Molekulárně-biologické metody (rodově a druhově specifická PCR a restrikční analýza amplifikované 16S rDNA s využitím 3 restrikčních enzymů) byly zvoleny pro ověření správnosti fenotypové identifikace izolátů bifidobakterií ze sbírky CCDM (Tábor, ČR). Molekulární metody byly zavedeny a optimalizovány s využitím typových a referenčních kultur bifidobakterií. Zvolené techniky umožnily identifikaci CCDM izolátů na úrověň druhů a poskytovaly shodné výsledky.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA202%2F02%2F1361" target="_blank" >GA202/02/1361: The use of magnetic carriers with immobilized selective ligands in DNA diagnostics</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    The 19th international ICFMH symposium FoodMicro 2004: New tools for improving microbial food safety and quality. Biotechnology and molecular biology approaches. 12-16th September 2004, Portorož, Slovenia

  • ISBN

    961-90346-3-5

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    151

  • Publisher name

    Slovenian Microbiology Society

  • Place of publication

    Ljubljana, Slovenia

  • Event location

    Portorož, Slovenia

  • Event date

    Sep 12, 2004

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article