TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010100" target="_blank" >RIV/00216224:14310/04:00010100 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

čeština

Original language name

TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA

Original language description

A multistep two-component system is involved in signal transduction in Arabidopsis and consists of a sensory histidine kinase, a phosphotransfer factor and a response regulator. Factors with histidine-containing phosphotransfer domains (AHPs) transfer the phosphoryl group from membrane localised receptor (histidine kinases) to effectors (response regulators) localised in the nucleus. Five AHPs have been identified in Arabidopsis. AHPs are small proteins (14,5 18 kDa), with a predicted acidic isoeletricpoint (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS. AHP5 comprises six exons and five introns. RNA was prepared using Trizol reagent from different plant tissues: leaves, roots, shoot, flowers, siliques and seedlings. Using RT-PCR we detected two products (AHP5s and AHP5l). Following sequencing, the second intron was found intact at position 211 bp in the longer product (AHP5l). Real time RT-PCR showed that the ratio between spliced and nonsp

Czech name

TISSUE SPECIFIC ALTERNATIVE SPLICING IN THE GENE FOR AHP5, A HISTIDINE-CONTAINING PHOSPHOTRANSFER PROTEIN FROM ARABIDOPSIS THALIANA

Czech description

A multistep two-component system is involved in signal transduction in Arabidopsis and consists of a sensory histidine kinase, a phosphotransfer factor and a response regulator. Factors with histidine-containing phosphotransfer domains (AHPs) transfer the phosphoryl group from membrane localised receptor (histidine kinases) to effectors (response regulators) localised in the nucleus. Five AHPs have been identified in Arabidopsis. AHPs are small proteins (14,5 18 kDa), with a predicted acidic isoeletricpoint (5.53.9) and a typical phosphotransfer domain, including the conserved sequence XHQXKGSSXS. AHP5 comprises six exons and five introns. RNA was prepared using Trizol reagent from different plant tissues: leaves, roots, shoot, flowers, siliques and seedlings. Using RT-PCR we detected two products (AHP5s and AHP5l). Following sequencing, the second intron was found intact at position 211 bp in the longer product (AHP5l). Real time RT-PCR showed that the ratio between spliced and nonsp

Type

D - Article in proceedings

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

VIII.Pracovní setkání biochemik a molekulárních biolog

ISBN

80-210-3321-5

ISSN

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e-ISSN

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Number of pages

2

Pages from-to

25-26

Publisher name

Masarykova univerzita v Brn

Place of publication

Brno

Event location

Konferenní centrum USKM Vinaská v Brn

Event date

Feb 3, 2004

Type of event by nationality

CST - Celostátní akce

UT code for WoS article

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