Activation of the DNA-binding ability of latent p53 protein by protein kinase C is abolished by protein kinase CK2

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010105" target="_blank" >RIV/00216224:14310/04:00010105 - isvavai.cz</a>

Alternative codes found

RIV/68081707:_____/04:00104694

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Activation of the DNA-binding ability of latent p53 protein by protein kinase C is abolished by protein kinase CK2

Original language description

Tumor suppressor protein p53 is one of the most important regulators of cell proliferation, differentiation and programmed cell death, triggering growth arrest and/or apoptosis in response to different cellular stress signals. The sequence-specific DNA binding function of p53 protein can be activated by several different stimuli modulating the C-terminal domain of this protein. The most likely physiological mechanism of p53 sequence-specific DNA binding activity is its post-translational modification, mainly phosphorylation of specific sites. Here we used non-radioactive electrophoretic mobility shift assay (EMSA) to show that C-terminal phosphorylation of p53 protein by cdk2/cyclin A at serine 315 and by PKC at serines 371, 376 and 378 can efficientlystimulate p53 binding to DNA in vitro as well as binding of C-terminal monoclonal antibody Bp53-10 recognizing the residue 371-380.

Czech name

Aktivace schopnosti vázat DNA u latentního proteinu přš pomocí protein kinasy C je potlačena protein kinasou CK2

Czech description

Tumorový supresorový protein p53 je důležitým regulátorem buněčné proliferace, diferenciace a programové buněčné smrti. Sekvenčně-specifická DNA vazebná funkce může být aktivována různými způsoby stimulace v C-koncové doméně proteinu, zejména fosforylacíspecifických míst. V této práci bylo použito stanovení využívající změnu elektroforetické mobility a piezoelektrický biosensor k demonstraci fosforylace serinových zbytků a dále k studiu vazby monoklonálních protilátek.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical Journal

ISSN

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e-ISSN

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Volume of the periodical

378

Issue of the periodical within the volume

11

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

9

Pages from-to

939-947

UT code for WoS article

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EID of the result in the Scopus database

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