The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010107" target="_blank" >RIV/00216224:14310/04:00010107 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

The immunosensors for measurement of 2,4-dichlorophenoxyacetic acid based on electrochemical impedance spectroscopy

Original language description

Electrochemical impedance spectroscopy was evaluated for the direct determination of herbicide 2,4-dichlorophenoxyacetic acid (2,4-D). Specific antibody against 2,4-D was immobilised onto different gold electrodes. Several methods of antibody immobilisation by covalent linkage to modified surface were studied. Self-assembled monolayers formed using thiocompounds as cystamine, 4-aminothiophenol (ATPh), 3,3'-dithiopropionic acid di(N-succinimidyl ester) (DTSP) and 11-mercaptoundecanoic acid (MUA) were chosen for the sensing surface activation. Three different sensor types were tested: screen-printed disc and finger-like structures and interdigitated array (IDA) electrodes produced by lithography. The measurements were carried out in a stationary arrangement and the reaction between hapten and the immobilised antibody was observed on-line. Changes of impedance parameters were evaluated and the best immobilisation technique (using 4-aminothiophenol) was chosen for further measurements. Imp

Czech name

Imunosensor pro stanovení 2,4-dichlorofenoxyoctové kyseliny využívající elektrochemickou impedanční spektroskopii

Czech description

Pro přímé stanovení herbicidu 2,4-D byla využita elektroimpedanční spektroskopie. Protilátky proti 2,4-D byly navázány na zlaté elektrody použitím různých monovrstev thiosloučenin (MUA, DTSP, cystamin, aminothiofenol). Byly využity sensory připravené sítotiskem i litografickou technologií. Byla sledována změna impedance sensorů v přítomnosti analytu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/OC%20518.30" target="_blank" >OC 518.30: Characterization of interactions of biomacromolecules with biolayers in different conformation states using piezoelectric biosensors</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

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Volume of the periodical

62

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

11-18

UT code for WoS article

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EID of the result in the Scopus database

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