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The rapid identification of European Armillaria species from soil samples by nested PCR

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010230" target="_blank" >RIV/00216224:14310/04:00010230 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The rapid identification of European Armillaria species from soil samples by nested PCR

  • Original language description

    New specific primers AR1 and AR2 were successfully used for the amplification of a specific part of internal transcribed spacer (ITS) of rDNA of Armillaria isolated from soil samples.The individual species were distinguished by restriction fragment length polymorphisms (RFLP) analysis with restriction endonucleases HinfI. For the first time, a method is described enabling the identification of Armillaria species within one day directly from soil samples without the need for previous isolation and cultivation of mycelium

  • Czech name

    Rychlá identifikace evropských druhů václavek z půdních vzorků pomocí metody nested PCR

  • Czech description

    V rámci studie byly připraveny specifické primery AR1 a AR2, které slouží k amplifikaci specifické části oblasti ITS v rDNA václavek. Různé druhy václavek byly rozlišovány pomocí RFLP amplifikovaného fragmentu. Námi vyvinutá metoda umožňuje v průběhu jednoho dne určit druh václavky z půdního vzorku pomocí nested PCR a to bez zdlouhavé kultivace.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EB - Genetics and molecular biology

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FEMS Microbiology Letters

  • ISSN

    0378-1097

  • e-ISSN

  • Volume of the periodical

    2004

  • Issue of the periodical within the volume

    237

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    6

  • Pages from-to

    105

  • UT code for WoS article

  • EID of the result in the Scopus database