Complex Study of Haloalkane Dehalogenase by Means of Electrophoretlcally Mediated Microanalysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00010517" target="_blank" >RIV/00216224:14310/04:00010517 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Complex Study of Haloalkane Dehalogenase by Means of Electrophoretlcally Mediated Microanalysis
Original language description
Halogenated aliphatic hydrocarbons constitute one of the largest groups of environmental pollutants. Haloalkane dehalogenases are a group of enzymes involved in the biodegradation of these compounds by catalysing cleavage of the carbon-halogen bond. Thestudy of the biochemistry of dehalogenation processes may help to understand and evaluate the potential for their degradation in nature. This work aims to develop Electrophoretically Mediated MicroAnalysis (EMMA)-based assays for the complex study of haloalkane dehalogenase. EMMA utilizes the different electrophoretic mobilities of enzyme, substrate and product to initiate reaction inside the capillary and to separate components from each other for final in-capillary quantitation. The Michaelis constants for different substrates, the inhibition constant for inhibitor 1,2-dichloroethane, substrate inhibition of 1,2-dibromoethane and the effect of temperature on enzymatic reaction of haloalkane dehalogenase from Sphingomonas paucimobilis
Czech name
Komplexní studie enzymu haloalkandehalogeansy metodou EMMA
Czech description
Jedná se o komplexní studii enzymu haloalkandehalogeansy metodou EMMA.
Classification
Type
D - Article in proceedings
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Book of abstract 14 th International Symposium on Capillary Electroseparation Techniques ITP 2004
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
103
Publisher name
Consiglio Nazionale delle Ricerche
Place of publication
ím
Event location
ím Itálie
Event date
Sep 12, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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