Electroanalytical determination of d(GCGAAGC) hairpin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011178" target="_blank" >RIV/00216224:14310/04:00011178 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electroanalytical determination of d(GCGAAGC) hairpin

Original language description

Hairpins (or hairpin-like structures) may play a major role in expansion events of triplet repeat expansion diseases (X syndrome, Huntington s disease, Friedreich s ataxia). The d(GCGAAGC) fragment has been found in the replication origins of phage fX 174 and herpes simplex virus, in a promoter region of an E.coli heat-shock gene, and in rRNA genes. The paper deals with the application of electrochemical methods to the determination of the DNA heptamer - d(GCGAAGC) which forms very stable hairpin structure in aqueous solutions. On mercury electrodes this hairpin provides voltammetric reduction signals of adenine and cytosine, and oxidation signals of guanine. Both signals have been studied by cyclic voltammetry (CV), linear sweep voltammetry (LSV), andelimination voltammetry with linear scan (EVLS) in dependence on pH, accumulation time, scan rate, and loop sequences. The EVLS in combination with the adsorptive stripping was employed to the determination of the detection limit (LD) o

Czech name

Elektroktroanalytické stanovení oligonukleotidu d(GCGAAGC)

Czech description

Elektroktroanalytické stanovení oligonukleotidu d(GCGAAGC)

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bioelectrochemistry

ISSN

1567-5394

e-ISSN

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Volume of the periodical

2004

Issue of the periodical within the volume

63

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

25-30

UT code for WoS article

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EID of the result in the Scopus database

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