Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011236" target="_blank" >RIV/00216224:14310/04:00011236 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Determination of isoflavones using high-performance liquid chromatography with electrochemical detection (HPLC- ED)
Original language description
A highly sensitive HPLC-ED was developed for the determination of isoflavones. The electrochemical behaviour of daidzein and genistein was studied on a carbon paste electrode by square wave voltammetry. The electrochemical results obtained were used forthe development of the HPLC-ED method. The HPLC-ED system used consisted of two solvent delivery pumps and an eight-channel CoulArray electrochemical detector. Isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15 M acetate buffer of pH 5.5 (solvent B) at a flow rate of 0.4 mL/min. The full scan of multi-channel coulometric detection was tested and an optimal potential at 450 mV was chosen for our purposes. The calibration curves werelinear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The relative standard deviation (R.S.D., n = 5) was 2.5 %. The detection limits for daidzein and genistein were 480 and 394 pg/mL (1.8 and 1.5 nM), respectively. Isoflavones extract
Czech name
Stanovení isoflavonů za použití HPLC s elektrochemickou detekcí
Czech description
Stanovení isoflavonů za použití HPLC s elektrochemickou detekcí
Classification
Type
D - Article in proceedings
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Book of Abstracts - 55th Annual Meeting of the ISE
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
433-433
Publisher name
International Society of Electrochemistry
Place of publication
Thessaloniki, Greece
Event location
Thessaloniki, Greece
Event date
Sep 19, 2004
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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