Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011226" target="_blank" >RIV/00216224:14310/04:00011226 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/04:00000330

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Determination of isoflavones in soybean food and human urine using liquid chromatography with electrochemical detection

Original language description

A highly sensitive high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) was developed for the determination of isoflavones. Electrochemical behaviour of daidzein and genistein was studied on carbon paste electrode (CPE)by adsorptive transfer stripping square wave voltammetry. The obtained electrochemical results were used for the development of HPLC-ED method. Furthermore, isoflavones were separated on an Atlantis dC18 column using a mobile phase consisting of acetonitrile (solvent A) and 0.15M acetate buffer of pH 5.5 (solvent B) at a flow rate 0.4 mL/min. A linear gradient profile (solvent B) was at 02 min 87%; 22 min 60%; 27 min 50%; 31 min 45%; 47 min 87%. Full scan of multi-channel coulometric detection was tested and optimal potential at 450mV was chosen for our purposes. Calibration curves were linear (daidzein R2 = 0.9993 and genistein R2 = 0.9987). The detection limit for daidzein/genistein was 480/394 pg/mL (1.8/1.5 nM) and per column 2.4/1

Czech name

Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí

Czech description

Stanovení isoflavon v sojových bobech a v lidské moi za použití HPLC s elektrochemickou detekcí

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Chromatography B

ISSN

1570-0232

e-ISSN

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Volume of the periodical

806

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

101-111

UT code for WoS article

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EID of the result in the Scopus database

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