Rapid-Resolution HPLC with Spectrometric Detection for the Determination and Identification of Isoflavones in Soy Preparations and Plant Extracts

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62156489%3A43210%2F07%3A00110002" target="_blank" >RIV/62156489:43210/07:00110002 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Rapid-Resolution HPLC with Spectrometric Detection for the Determination and Identification of Isoflavones in Soy Preparations and Plant Extracts

Original language description

A rapid-resolution HPLC/UV-VIS DAD separation method (which takes < 1 min) for the determination and identification of genistin, genistein, daidzein, daidzin, glycitin, glycitein, ononin, formononetin, sissotrin and biochanin A in fmol quantities in submicroliter sample volumes was optimized. A linear gradient elution (0 min 22% B, 1.0 min 80% B, 1.4 min 100% B, 1.8 min 22% B) using a mobile phase containing 0.2 % (v/v) acetic acid (solvent A) and methanol (solvent B) was applied on a Zorbax SB C-18 column (1.8 mu m particle size) at 80 degrees C. The method was verified using samples of bits of soy and methanolic extracts from Trifolium pratense, Iresine herbstii and Ononis spinosa plants. Pseudobaptigenin glucoside, irilone, prunetin, texasin, tlatlancuayin and other isoflavones, in addition to aglycones of isoflavones and their beta-glucosides and malonyl and acetyl derivatives, were identified by UV-VIS DAD and electrospray mass spectrometric (ESI-MS) detection in the extracts.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA525%2F07%2F0338" target="_blank" >GA525/07/0338: Isoflavonoids in algae and cyanobacteria</a><br>

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and bioanalytical chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

389

Issue of the periodical within the volume

7-8

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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