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Magnetic hydrophilic methacrylate-based polymer microspheres designed for PCR-ready DNA isolation

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011381" target="_blank" >RIV/00216224:14310/04:00011381 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Magnetic hydrophilic methacrylate-based polymer microspheres designed for PCR-ready DNA isolation

  • Original language description

    Newly designed magnetic nonporous poly(2-hydroxyethyl methacrylate-co-ethylene dimethacrylate) - P(HEMA-co-EDMA), poly(glycidyl methacrylate) - PGMA and P(HEMA-co-GMA) microspheres carrying carboxyl groups were used for isolation of genomic DNA.Microspheres were prepared by radical dispersion copolymerization of respective monomers in the presence of colloidal iron oxides. DNA isolated from bacterial cells of Bifidobacterium longum by magnetic microspheres was used for PCR testing.

  • Czech name

    Magnetické hydrofilní polymerní mikročástice na bázi methakrylátů vyvíjené pro izolaci DNA přímo použitelnou v PCR

  • Czech description

    Nově vyvíjené magnetické neporézní mikročátice na bázi poly(2-hydroxyethyl methakrylát-ko-ethylen dimethakrylátu) -P(HEMA-co-EDMA), poly(glycidyl methakrylátu) - PGMA a P(HEMA-co-GMA), obsahující karboxylové skupiny,byly použity pro izolaci genomové DNA.Mikročástice bylybyly připraveny radikálovou disperzní kopolymerizací monomerů v přítomnosti koloidních oxidů železa.Pro pCR aplikace byla použita DNA izolovaná z bakteriálních buněk Bifidobacterium longum.

Classification

  • Type

    D - Article in proceedings

  • CEP classification

    EE - Microbiology, virology

  • OECD FORD branch

Result continuities

  • Project

    <a href="/en/project/GA202%2F02%2F1361" target="_blank" >GA202/02/1361: The use of magnetic carriers with immobilized selective ligands in DNA diagnostics</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2004

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Article name in the collection

    Book of Abstarcts

  • ISBN

  • ISSN

  • e-ISSN

  • Number of pages

    1

  • Pages from-to

    "F21"

  • Publisher name

    Royal Flemish Chemical Society

  • Place of publication

    Bruges

  • Event location

    Bruges

  • Event date

    Feb 4, 2004

  • Type of event by nationality

    WRD - Celosvětová akce

  • UT code for WoS article