Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F11%3A00053209" target="_blank" >RIV/00216224:14310/11:00053209 - isvavai.cz</a>
Alternative codes found
RIV/61389013:_____/11:00359412 RIV/00216305:26310/11:PU93907
Result on the web
<a href="http://dx.doi.org/10.1016/j.eurpolymj.2011.02.007" target="_blank" >http://dx.doi.org/10.1016/j.eurpolymj.2011.02.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.eurpolymj.2011.02.007" target="_blank" >10.1016/j.eurpolymj.2011.02.007</a>
Alternative languages
Result language
angličtina
Original language name
Streptavidin-modified magnetic poly(2-hydroxyethyl methacrylate-co-g-lycidyl methacrylate) microspheres for selective isolation of bacterial DNA.
Original language description
The dispersion copolymerization of 2-hydroxyethyl methacrylate (HEMA) with glycidyl methacrylate (GMA) in a toluene/2-methylpropan-1-ol mixture in the presence of Fe3O4 nanoparticles coated with oleic acid, produced monodisperse magnetic poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) (P(HEMA-GMA)) microspheres. Oxirane groups of the microspheres were ammonolyzed and then functionalized with streptavidin using cyanuric chloride. The final product contained 0.67 mg of streptavidin per gram ofwet magnetic P(HEMA-GMA) microspheres. The microspheres were characterized by elemental analysis, scanning electron microscopy, IR, UV?VIS and atomic absorption spectroscopy The streptavidin-modified magnetic P(HEMA-GMA) microspheres were used for immobilization of biotinylated DNA and subsequent selective isolation of target DNA from complex samples using DNA/DNA hybridisation.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CD - Macromolecular chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
European Polymer Journal
ISSN
0014-3057
e-ISSN
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Volume of the periodical
47
Issue of the periodical within the volume
5
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
1090-1096
UT code for WoS article
000290701000027
EID of the result in the Scopus database
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