Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F04%3A00011440" target="_blank" >RIV/00216224:14310/04:00011440 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Capillary Electrophoresis of Bacteriophage 812 Proteins Combined with MALDI Mass Spectrometry

Original language description

The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification.

Czech name

Kapilární elektroforéza proteinů bakteriofága 812 kombinovaná s MALDI-hmotnostní spektrometrií

Czech description

The alarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was used for the protein separation followed by tryptic digestion and ESI or MALDI analysis.Additionally, we have proposed a new approach to the analysis of the phage proteins, especially low abundant proteins, such as lytic enzymes. Protein and peptide separations were performed with capillary electrophoresis (CE) and a new universal interface has been developed to couple CE with MALDI MS. A method of protein microdigestion on MALDI target is being studied and will be used for protein identification. Anotace anglicky: Thealarming increase in antibiotic-resistant bacteria has renewed interest in bacteriophage therapy of various human infections, such as postoperative staphylococcal wound infections or anthrax. In this work, SDS gel electrophoresis was use

Type

D - Article in proceedings

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/GA203%2F03%2F0515" target="_blank" >GA203/03/0515: Integrated genome and proteome analysis of therapeutically important bacteriophages by combination of electrophoresis and mass spectrometry</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Final Program and Book of Abstracts of 17th International Symposium on Microscale Separations and Capillary Electrophoresis

ISBN

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ISSN

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e-ISSN

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Number of pages

2

Pages from-to

83-84

Publisher name

HPCE

Place of publication

Salzburg, Austria

Event location

Salzburg, Austria

Event date

Feb 8, 2004

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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